Insertion junction: CLIP2.026191_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026191
Chromosome:chromosome 16
Location:6788687
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g672250 MPA13 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTGCCAAGCCTCGTGGTCGATTCGTGTACCGATTTGCGCTGCTCCTGC
Internal bar code:TAGTACGTTCCAGTCTGACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3292
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCGCTAACAGACATTC
Suggested primer 2:CACCCAATCCCTCACCTCAC