Insertion junction: CLIP2.026222_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026222
Chromosome:chromosome 6
Location:2389965
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268501 (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACAACACACGCACACGCACACGCACATGCGTTAATGACATCCG
Internal bar code:AGAAGAATCAGTTGAGTAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2014
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCCACCTCCAGAAACA
Suggested primer 2:TGCTGCAAAGGTAGGAGTGG