| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.026258 |
| Chromosome: | chromosome 4 |
| Location: | 3154184 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226100 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTGGAGCACGCGGGCGCGCCTGCCGTCGCCTCAAGCGGCGGCCGAC |
| Internal bar code: | CGTTAGTGCGGTGATAATTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2298 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAAGCCACAAACGTCTGG |
| Suggested primer 2: | GCACTCTCCCCACCAGTTAC |