| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.026290 |
| Chromosome: | chromosome 3 |
| Location: | 8101970 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201050 | BSD2,ZNJ2 | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Bundle-sheath defective-2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCACCGGCTTCCAGAGCGCTTTCTAACAATGCGTTGAGAGGACTGGAG |
| Internal bar code: | TCTACAGGCGAGCCGGGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2269 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTCCTCAACGCTTAGC |
| Suggested primer 2: | GCTGAATTTCCATCGTCCGC |