Insertion junction: CLIP2.026294_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.026294
Chromosome:chromosome 8
Location:1892631
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367600 ASL1,CYSL,CYSM,OASTL1 (1 of 3) 2.5.1.47 - Cysteine synthase / OAS sulfhydrylase; O-acetylserine (Thiol)-lyase/cysteine synthase 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTGCTGCCCAGCTTTGGCGAGCGCTACCTGTCCACGGGTGAGAGTGC
Internal bar code:AGAGTCTTGTGCCCGTATTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3775
LEAP-Seq percent confirming:96.0784
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCATCTACACGGCATTGC
Suggested primer 2:GTGTCTCTTCGCAAAGCACG