| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.026300 |
| Chromosome: | chromosome 12 |
| Location: | 3820393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514800 | (1 of 2) K06287 - septum formation protein (maf) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCACCAGACGAGCGCACACACGCCGCGTGACGGCTACCGGCGGCGCT |
| Internal bar code: | CCGGGGTAGCCGAAGTACCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2241 |
| LEAP-Seq percent confirming: | 85.1852 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGGCTTCACTTACGAA |
| Suggested primer 2: | ATAGAACACCTCGCCCTCCT |