| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026404 |
| Chromosome: | chromosome 7 |
| Location: | 3605748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336950 | PHO2,PHOA2,PHOA | Starch phosphorylase 2, PhoA; (1 of 2) 2.4.1.1 - Glycogen phosphorylase / Polyphosphorylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGTTCATTCGCCATACCCATGACCGACAGGGTGTCGAGGAGGGTCA |
| Internal bar code: | GGTAGTTTATACATGAGCCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1949 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTTCTTGACCTTCACCT |
| Suggested primer 2: | GGGTCAGATAGAATCGGCCG |