| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.026408 |
| Chromosome: | chromosome 10 |
| Location: | 3923774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446650 | HEL46,MER | (1 of 1) K15271 - ATP-dependent DNA helicase HFM1/MER3 [EC:3.6.4.12] (HFM1, MER3); conserved protein with DEAD/DEAH box helicase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCGGCGCTGAAGCCTCCGCAGCTTAGGAGTGAGGGCCAGAGTTGCTG |
| Internal bar code: | ATGATAGCTATTGTAATCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2019 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGCTTCGGTAAGTGGT |
| Suggested primer 2: | TCCTAGATGCCTGGAGCTGT |