Insertion junction: CLIP2.026424_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026424
Chromosome:chromosome 14
Location:3004021
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g627650 FAP392 Flagellar Associated Protein 392 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGAAATGACTGCGTACGTGGCTTGGGCGCGCATCACACAGGTCGGGT
Internal bar code:AGGTCAAGCTACTATATACAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1102
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCCCGCTACTCTAACCT
Suggested primer 2:AACCCCAAACCGGTCTTGTT