Insertion junction: CLIP2.026435_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.026435
Chromosome:chromosome 9
Location:493480
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g749047 (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGAACCTGCACCTGCTTCTGGACTGCACAGCCCGGCGCGTATGTGG
Internal bar code:TTTGAACGGCTGGCTACAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:955
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCTCCAGTGTTCATCG
Suggested primer 2:CCTCGGTTATATGCAGCGGT