| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026445 |
| Chromosome: | chromosome 7 |
| Location: | 6396338 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357600 | 5'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTTAACAATGGAAGACTGCGGCCGCGTGGTCAAGTATGTTATAGAC |
| Internal bar code: | TACTACAGTCTTTCTCGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAACGTTTGACCTTGGC |
| Suggested primer 2: | CGCTCCGGCTGGATATTCTT |