| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.026447 |
| Chromosome: | chromosome 13 |
| Location: | 2832652 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582900 | (1 of 77) IPR001623 - DnaJ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCACGCGGTGGCAGAGGTGGTCATGCAGAGGTCAAGGGCAGGTGTGG |
| Internal bar code: | TATGTAACCTGTGACTCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4884 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGAGGCAACAGTCGAA |
| Suggested primer 2: | TGCATCGCATCCACTGTCTT |