| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.026452 |
| Chromosome: | chromosome 10 |
| Location: | 3168481 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441800 | (1 of 4) PF05066 - HB1, ASXL, restriction endonuclease HTH domain (HARE-HTH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCCGCGCCTCACGGTCCTGGCGCAACGCGGGCAGCGACGCCGAAGAT |
| Internal bar code: | GTTGGGAATTTAACGGACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCTCCCGCCATTCTCC |
| Suggested primer 2: | CTTGCCGTTGAGATGGGTCT |