Insertion junction: CLIP2.026453_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.026453
Chromosome:chromosome 17
Location:262982
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697650 (1 of 1) K01354 - oligopeptidase B (ptrB) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGGGAGGGGTAGCCGTCCGCGGACAGCTGGGTGAATGCATAAAACA
Internal bar code:TGAGTAGGGAAGACGAAGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTACTACACGCGGCAGG
Suggested primer 2:CATACGCGCACACACAGATG