Insertion junction: CLIP2.026465_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.026465
Chromosome:chromosome 2
Location:2233080
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089850 (1 of 1) IPR000969//IPR013719 - Structure-specific recognition protein // Domain of unknown function DUF1747 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAAGACAGGAGAGGGGATTTGTAAATACCAGCCCAACTGAACCAACTG
Internal bar code:GCCTATCTATCGCGGGTGATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCTAGTTTCGCTTGGTT
Suggested primer 2:CAACGCCAAGTCAAGTGTGG