| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026488 |
| Chromosome: | chromosome 3 |
| Location: | 6019643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCCGGTGGAAGTAGGTCTGGGAGTTTTGGAACAGGAGCGGATGGGC |
| Internal bar code: | CGAGAACACCAATCGGGGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4196 |
| LEAP-Seq percent confirming: | 93.9189 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 148 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAGGGTAGGCAATTGGC |
| Suggested primer 2: | CGTCAACGCACAAGAAAGCA |