Insertion junction: CLIP2.026489_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026489
Chromosome:chromosome 5
Location:1073793
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACGCTGCCACCACACAAGCTAGCACACACATGCTGCCACACACAACC
Internal bar code:GCTTTTTAGCGGCGCAGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:577
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTGCCATGGTGTGAGAT
Suggested primer 2:AGCAGCTATTGGCCACCATT