| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.026519 |
| Chromosome: | chromosome 13 |
| Location: | 2872738 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583200 | NAT5 | N-acetyltransferase related to GCN5; (1 of 6) PF02656 - Domain of unknown function (DUF202) (DUF202) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCACGCAGGGTGCAGTCGGGACACAGTATGCTAGCCGTCGAGCCTCTG |
| Internal bar code: | TCTTACGCTTGTTGAAATGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAAATGCGGCTTGCCTT |
| Suggested primer 2: | CAAACTGTTTGGCCCAGTCG |