| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.026571 |
| Chromosome: | chromosome 16 |
| Location: | 3745822 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683437 | (1 of 1) K16278 - E3 ubiquitin-protein ligase HOS1 [EC:6.3.2.19] (HOS1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGGCGCGCGACCACTCGGGGTAGGAGCCGAAGCGGCCGGTGGCGGC |
| Internal bar code: | GGTACTAATTACAACAGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2468 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGGAGGTGGTGTGTGC |
| Suggested primer 2: | CGCAAGCAAACTCAAGCAGT |