Insertion junction: CLIP2.026578_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.026578
Chromosome:chromosome 17
Location:2818729
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g718400 (1 of 1) IPR001810//IPR011050 - F-box domain // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCAGCACCGCAGCTGATCATGCGCGCGGGGCTGCACAAGGACGTGCG
Internal bar code:AGTCGTGGTTATTGTGTTCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1336
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCGTCGTCCTCATCACT
Suggested primer 2:GCAAAAGGAGAGCCCAGGAT