| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026585 |
| Chromosome: | chromosome 16 |
| Location: | 1353447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651850 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGATACTGTAAAGGCGCCCGTCCGAGCCCTCCGCCTGATAACTGGCTG |
| Internal bar code: | AGAAGAATCGTCTCATGCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5625 |
| LEAP-Seq percent confirming: | 97.1014 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTTGCCGTCATCACTTT |
| Suggested primer 2: | TCAACGACCAGTAGCTGCAG |