Insertion junction: CLIP2.026594_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026594
Chromosome:chromosome 5
Location:1370790
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234636 PFLAE,PFA1,PFLA1 Pyruvate formate lyase activating enzyme 1; (1 of 1) 1.97.1.4 - [Formate-C-acetyltransferase]-activating enzyme / S-adenosyl-L-methionine-cleaving) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAATTTAACCCCACCCCACCCCACCTGGAACACGGTGGACACAAAGTGT
Internal bar code:AGGGTATCGTTGTTAAAAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:341
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTCCTGGTGTTTGTGCA
Suggested primer 2:GTGGTGGTTAGGGTTAGGGC