Insertion junction: CLIP2.026609_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026609
Chromosome:chromosome 3
Location:3910509
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g170850 MMP19 Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGTCGGGTCCGCCAGCCTGCGGGTGCAGCTGCCAGAGGAGGTCGGGC
Internal bar code:CCATGTAGATAATTACTCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACGTGACTGTACGAACA
Suggested primer 2:CCGCATGCATTAGACGCTTC