Insertion junction: CLIP2.026610_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026610
Chromosome:chromosome 10
Location:4014226
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g447500 KIN12-3,KIN16B,KIN16-2 Kinesin motor protein; (1 of 1) K10392 - kinesin family member 1 (KIF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTGTGCGCGCGGCACTTCATTGCATGGAGCGCCTGCTTCCAATTTGA
Internal bar code:AGACAAACAAAGACCCTAGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1573
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCAAGCTGACGTGTCTGT
Suggested primer 2:GTCATTGTCTCGATTGCGCC