| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.026617 |
| Chromosome: | chromosome 6 |
| Location: | 8049568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305050 | EEP5,CGL45 | expressed protein Conserved in the Green Lineage; (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTATAATCAAATTCCACAGAAGAAGTTACTCGAACACCAGCCTCGCG |
| Internal bar code: | CCGCTGAAGGCGCAGAGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1296 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGTGGAGCGAGAGTG |
| Suggested primer 2: | TGTCAAAGCCAACATGCACG |