| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.026630 |
| Chromosome: | chromosome 10 |
| Location: | 5453765 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457500 | CKIN beta,SNRKB1 | regulatory subunit beta of snRK1 complex in Chlamydomonas; (1 of 1) K07199 - 5'-AMP-activated protein kinase, regulatory beta subunit (PRKAB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATGCCAAGTACAGTCCACGCCACGCCATTCCACCTCACACACCTGG |
| Internal bar code: | AAGGGTCCTGAGTATGGTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2772 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCGGCATGTCCTCTATC |
| Suggested primer 2: | CCGCGTCCGATAACCATACA |