Insertion junction: CLIP2.026647_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.026647
Chromosome:chromosome 12
Location:8944572
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g545600 (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGAGAGTTTATCGCCGCTCCCCCCCTAACCGCAGATCTTCCCCCGGC
Internal bar code:ACGCGAGAGCTTTGTTCGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1132
LEAP-Seq percent confirming:89.6552
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTCGCTATCTCCGCATG
Suggested primer 2:TCATCCCAAATCCACCTGCC