| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026654 |
| Chromosome: | chromosome 14 |
| Location: | 648414 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612150 | MOT18,PAP10 | Class-II RNA nucleotidyl transferase 10; (1 of 1) K14079 - poly(A) RNA polymerase GLD2 [EC:2.7.7.19] (PAPD4, GLD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAACAGGGAGGCTCGGCAGTGGCGCACCGCCTGCCTCTCCCTCAGCCC |
| Internal bar code: | TCAATCAGACTTTAAGATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGAGCCGTCAACCCTGA |
| Suggested primer 2: | AGTGGAAGAAGTGCGTAGGC |