| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026659 |
| Chromosome: | chromosome 1 |
| Location: | 2324895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013000 | (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATACCTACGCCGGTTACCGAGCTCCGCGTTGGGCGATGTTGCCCCAG |
| Internal bar code: | GGTGCATCCGATTTTACCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5000 |
| LEAP-Seq percent confirming: | 86.9919 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATGAGGAGGTGGAGGT |
| Suggested primer 2: | ATGCTGTGGGTCTGTCATGG |