Insertion junction: CLIP2.026690_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.026690
Chromosome:chromosome 7
Location:774253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g317908 (1 of 1) K04485 - DNA repair protein RadA/Sms (sms, radA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCACAACCATTTCCTCGACCATGCAGCCCCATCCCATCCCATCCAG
Internal bar code:GGCCTGAATCAAGTCTGAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCGCATTCGTCACACTC
Suggested primer 2:ACATACCACGCGTGTTTCCT