Insertion junction: CLIP2.026693_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.026693
Chromosome:chromosome 3
Location:8259974
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g199700 PGM11 (1 of 18) PF00300 - Histidine phosphatase superfamily (branch 1) (His_Phos_1); Phosphoglycerate mutase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGCGCGGGCGCGGGTGTCGCTATGTGCACAGAAGCCTCTCAGGTTCA
Internal bar code:CTTCGCGCCTCGAGCTTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2928
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTACTGCTTCTGCTGCTT
Suggested primer 2:TTCAACGTGGCAGTCCTACC