Insertion junction: CLIP2.026702_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.026702
Chromosome:chromosome 2
Location:3240035
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097150 AGG5 (1 of 12) PF04749 - PLAC8 family (PLAC8); Aggregation 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACACACACACACACACACACGCCCCCAGGACGCGCGTGAGATTATGA
Internal bar code:ATGACGTCTTATACCTTTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1114
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGTAGTTACCTCCGCCA
Suggested primer 2:TACCTGCGCAAGAAGTACGG