Insertion junction: CLIP2.026712_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.026712
Chromosome:chromosome 6
Location:3059509
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g274950 (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGGGCAGGGCAGTACGCGCTTGCTGCTGTCCTCGCATCCTCAACCCA
Internal bar code:AGGGGAGCTGCTACCTGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:71
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGTCACAACTGGCCATGT
Suggested primer 2:TTGCAGCAGATCAAGGGGAG