Insertion junction: CLIP2.026768_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026768
Chromosome:chromosome 1
Location:1882599
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800035 (1 of 2) PTHR12436:SF4 - LEUKOCYTE RECEPTOR CLUSTER MEMBER 8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAATCCAACCCCGCACCAAAGCAAATTCTACCTTCCCCCATCTCCTCA
Internal bar code:CTGCCCCGGCGCCCTGTCAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:39.2857
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAATTCCCCATGCACCA
Suggested primer 2:TCATCACCCTCACACACACG