| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.026774 |
| Chromosome: | chromosome 8 |
| Location: | 910645 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361800 | RPC31 | (1 of 1) PF11705 - DNA-directed RNA polymerase III subunit Rpc31 (RNA_pol_3_Rpc31); DNA-directed RNA polymerase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACGGTGCTATGCGACATGACGCATGTGGCAGATGCAGGCCCGGCAC |
| Internal bar code: | AAATTTCCTTTGGCTAAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2592 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTTCTTCGTTGCAACTG |
| Suggested primer 2: | CGGTATCAGGGTCATCACCG |