Insertion junction: CLIP2.026788_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026788
Chromosome:chromosome 3
Location:5891903
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188600 RBL11 Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCAGCCACCTGCACGCAGGTGTTGAACGGCCGTGCGTGGCGCCGGGC
Internal bar code:TCTTAACGCGGCCAACTGAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3024
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAATGTCACTGCCATGCTCG
Suggested primer 2:ATTATCGTGGGGGCGTCATC