| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.026798 |
| Chromosome: | chromosome 7 |
| Location: | 4596660 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344200 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACCTGCCATACCCAGGCACAGCCCCGCCGCCGCCAGAAGTGTCGCC |
| Internal bar code: | TCTCATCTGGTGATATTATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGTGTGTGTGTGCGTG |
| Suggested primer 2: | TCATCGTCGTCAAACACCGT |