| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026811 |
| Chromosome: | chromosome 9 |
| Location: | 2141433 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395800 | (1 of 1) PF05907 - Eukaryotic protein of unknown function (DUF866) (DUF866) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATACAAGTTCCACTTCTGCTCATCTGAACTTACAGGCATTGTATCAG |
| Internal bar code: | CTTGTAGTAGAAACTTAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2129 |
| LEAP-Seq percent confirming: | 69.697 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGTTGGCAAGACAAG |
| Suggested primer 2: | ACGCACTGTGAATCTGTCGT |