| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.026821 |
| Chromosome: | chromosome 4 |
| Location: | 2261276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACAAGACTCCTTAAGTACCAGTCTCTAGTATTGTGACATAGAACGA |
| Internal bar code: | TAGTCAGTAGTAATATAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCAGTTCGCCGAGGATC |
| Suggested primer 2: | ACTTGCGGTTTGGGACCTAG |