Insertion junction: CLIP2.026821_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.026821
Chromosome:chromosome 11
Location:1208937
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATGTTCCGTCCACCTCACAACGTCACCGCTTCTTCACACCGCCTCAC
Internal bar code:CGAAAGAGTTTCGTGCCCGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:81
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTAGTGGTGGAGGAGGAG
Suggested primer 2:CCTGGTCGTGCAAGGAATCT