| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.026837 |
| Chromosome: | chromosome 13 |
| Location: | 5001476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606101 | (1 of 2) PTHR19959//PTHR19959:SF147 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACATCACACATCACAGAACGGCGCTGGTCATCTACGGCGAGCG |
| Internal bar code: | ATATGATCCAGTACAAGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3153 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCCGGGGTGATGATTAA |
| Suggested primer 2: | ATGAGTGTGTGGCATTCGGT |