Insertion junction: CLIP2.026855_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026855
Chromosome:chromosome 2
Location:2117351
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089200 HLM4,SET3 (1 of 1) PTHR22884//PTHR22884:SF370 - SET DOMAIN PROTEINS // HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-9 SPECIFIC SUVH5-RELATED; Histone-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCGACGTCGCTTGTTGACGGCAGCGCCGGCGGCACCCCCGCCGCCGC
Internal bar code:GCTTCCTGATGTTGGGGCCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1242
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGCCCCCATGTTTTTGC
Suggested primer 2:AGGAGTGGTTGATGAAGCGG