Insertion junction: CLIP2.026859_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.026859
Chromosome:chromosome 7
Location:5874327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353750 MMP10 Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin CDS
Cre07.g800866 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACGTCACCTACGCCAACAAGGTGCATTCAGTAGTTGTGCGTGTGTGT
Internal bar code:CGTATGCATTAGTTGGATCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:574
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGTTGTGGTTGTCGTTG
Suggested primer 2:CACAGCTCTGACTCCTGGTG