| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.026867 |
| Chromosome: | chromosome 11 |
| Location: | 534385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTACAGCGCAGCCTTGGAGGACGGCCCCGCCGCCGCCGCGGAGGCGGC |
| Internal bar code: | TAGGTACCAGGTCATGGAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4847 |
| LEAP-Seq percent confirming: | 89.5833 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACAGACGCCTTCAGTGA |
| Suggested primer 2: | AGGTGATGGTGTTGAGGCTG |