| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.026876 |
| Chromosome: | chromosome 9 |
| Location: | 3061435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393876 | (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACACTTGCGGCTCCTGCCGATTCCGCCCATCCTCTGCGTGCAGGCA |
| Internal bar code: | TTATACATCTCAAGTACCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2147 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACTGAAATGGCTATGCG |
| Suggested primer 2: | GGGTCTGGTTGTGGTTGTGA |