| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.026888 |
| Chromosome: | chromosome 12 |
| Location: | 4523596 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521100 | POB5 | Proteome of basal body 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGATTTCAAGCAGCCTCACTCGGTGGCGGCGTCGGCGGCGATGGCGG |
| Internal bar code: | GCTCCCGTCCTGGTCGGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4297 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGGCCCTCATGTACTTT |
| Suggested primer 2: | AGGGACATGCTTACGGTGTG |