Insertion junction: CLIP2.026899_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026899
Chromosome:chromosome 8
Location:129826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358535 3'UTR
Cre08.g800882 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAAGGGACGTTTTGTGGTTGCGTGCGTGCGGGCAGAGTGCGCGTGGA
Internal bar code:TGCAACATATATACTAGATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4213
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTCTCAAGTCGTGCCAA
Suggested primer 2:CATCGAGATGGTACCCTGGC