Insertion junction: CLIP2.026925_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026925
Chromosome:chromosome 8
Location:2439060
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g371957 RAPTOR1,RAPTOR Regulatory-associated protein of TOR; (1 of 1) K07204 - regulatory associated protein of mTOR (RAPTOR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAAAGCGGTACAGGATGCACTGAAGAGTGGGGTGACATTCATGATTAC
Internal bar code:CGCATCCGTGACCACGTTTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:226
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTGGATGTACGCCCTCC
Suggested primer 2:TAGGGGTGGGTGTAGATGGG