Insertion junction: CLIP2.026944_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026944
Chromosome:chromosome 2
Location:3073635
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095900 ROC114,ROC108 Rhythm Of Chloroplast 114; (1 of 13) PF00646 - F-box domain (F-box) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAGAAGTGGTTTCCGTGGTCGACGGTTCATCTAGGCAGGCCATCACAA
Internal bar code:TGCGATCTGATTTTGCGCTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:648
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCAACCAGTTGCACAA
Suggested primer 2:AGTTCCCCCGCATCAAGAAG