| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.026948 |
| Chromosome: | chromosome 12 |
| Location: | 8905709 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545850 | FAP363 | Flagellar Associated protein 363; (1 of 1) PTHR19375:SF152 - HEAT SHOCK 70 KDA PROTEIN COGNATE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGTGGAGGCATAGCCAGGTACAGTAGTATAGCTGGTGCAGTAGGAC |
| Internal bar code: | GTTTGCGGACTTTACAGGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 84.4828 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCTCACGTGTTTGTGG |
| Suggested primer 2: | AAGCATAAGGACCAACGGGG |